8YLF

Crystal structure of Burkholderia thailandensis MftR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	62.389, 62.389, 77.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.930 - 2.010
R-factor	0.2175
Rwork	0.217
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house Se-SAD model
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.040
High resolution limit [Å]	2.010	2.010
Number of reflections	11986	549
<I/σ(I)>	53	3.8
Completeness [%]	99.6	94
Redundancy	10.3	8.2
CC(1/2)	0.993	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	imidazole, sodium citrate