8YL3
Crystal Structure of Human Rab23 in Complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-20 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.911, 57.154, 63.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.290 - 1.200 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.18860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.790 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.300 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.049 | 0.717 |
| Rmeas | 0.055 | 0.833 |
| Rpim | 0.024 | 0.416 |
| Total number of observations | 233754 | |
| Number of reflections | 49198 | 2427 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 3.8 |
| CC(1/2) | 1.000 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Sodium acetate tribasic pH 5.0, 0.2M MgCl2, 27.5% PEG 6000 |
