8YKE
Structure of two consecutively arrayed Rib domains (Rib8-9) from surface adhesin of Limosilactobacillus reuteri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2023-05-24 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 76.085, 76.085, 51.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.060 - 1.800 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.912 |
| Data reduction software | SADABS |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.100 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.158 | 0.631 |
| Number of reflections | 14648 | 2126 |
| <I/σ(I)> | 25.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M NH4OAc, 0.1M Tris pH 8.5, 25% PEG 3350 |
