8YKC

Crystal structure of adenosylcobinamide kinase / adenosylcobinamide phosphate guanylyltransferase complexed with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	55.920, 73.960, 130.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.212 - 1.921
Rwork	0.221
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.678
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.212	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.050
Number of reflections	41759	46479
<I/σ(I)>	34
Completeness [%]	99.1
Redundancy	13
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	.2 M ammonium chloride, pH 6.3 and 20 % w/v PEG 3350