8YK7
Structure of Rib domain from surface adhesin of Limosilactobacillus reuteri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2022-09-24 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 76.274, 76.274, 23.326 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.910 - 1.350 |
| R-factor | 0.12901 |
| Rwork | 0.127 |
| R-free | 0.16725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.438 |
| Data reduction software | SADABS |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.061 | 0.400 |
| Number of reflections | 17478 | 3451 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.01M ZnSO4, 0.1M MES pH 6.5, 20% PEGMME 550, protein is premixed with 3mM CuCl2 |
