8YJX

Crystal structure of penicillin-binding protein 2 (PBP2) from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-02-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	119.867, 119.867, 274.058
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.790 - 2.950
R-factor	0.2369
Rwork	0.235
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.950	2.950
Number of reflections	49100	2461
<I/σ(I)>	12.2	0.6
Completeness [%]	99.9	99.9
Redundancy	4.9	4.8
CC(1/2)	0.989	0.296

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	NaCl, Tris