8YJJ

Crystal structure of xylanase from Trichoderma longibrachiatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	40.476, 39.085, 56.640
Unit cell angles	90.00, 110.33, 90.00

Refinement procedure

Resolution	27.230 - 1.900
R-factor	0.1793
Rwork	0.177
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xyp
RMSD bond length	0.002
RMSD bond angle	0.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.123	0.262
Number of reflections	22849	644
<I/σ(I)>	16.23
Completeness [%]	96.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	MES, ammonium sulfate, magnesium chloride