8YJI

Room temperature structure of xylanase from Trichoderma longibrachiatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	299.15
Detector technology	CCD
Collection date	2019-05-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	41.097, 39.272, 56.749
Unit cell angles	90.00, 110.25, 90.00

Refinement procedure

Resolution	31.600 - 2.100
R-factor	0.1959
Rwork	0.193
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xyp
RMSD bond length	0.003
RMSD bond angle	0.610
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.141	0.466
Number of reflections	9843	464
<I/σ(I)>	20.83
Completeness [%]	95.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	MES, ammonium sulfate, magnesium chloride