8YJG

Crystal structure of the nucleotide-binding domain of Candida glabrata Hsp90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 43 2 2
Unit cell lengths	73.942, 73.942, 108.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.570 - 1.900
R-factor	0.2046
Rwork	0.203
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.444
Number of reflections	24325	1193
<I/σ(I)>	48.2	7.3
Completeness [%]	99.4	100
Redundancy	7.6	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M HEPES pH7.5, 70% (v/v) 2-methyl-2,4-pentanediol