8YJ3
N17.1.2 recognition of NRAS neoantigens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.228, 66.113, 138.590 |
| Unit cell angles | 90.00, 97.81, 90.00 |
Refinement procedure
| Resolution | 47.621 - 3.503 |
| R-factor | 0.2608 |
| Rwork | 0.258 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.621 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.206 | |
| Number of reflections | 148044 | 23156 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 97.7 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.953 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8mg/ml, 0.2 M MgCl2, 0.2M NaNO3, 14-18% PEG3350 |
