8YIM
Crystal Structure of Human Rab23 in complex with GMPPNP (1.35 Angstroms Resolution)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.523, 60.523, 93.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.880 - 1.350 |
| R-factor | 0.1513 |
| Rwork | 0.150 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.680 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.880 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.133 | 2.087 |
| Rmeas | 0.141 | 2.260 |
| Rpim | 0.046 | 0.862 |
| Number of reflections | 44414 | 2185 |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9 | 6.8 |
| CC(1/2) | 0.997 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Imidazole pH 8.0, 0.2M NaCl, 0.4M sodium phosphate monobasic/1.6M potassium phosphate dibasic |
