8YHZ
The co-crystal structure of the Fab fragment of Ab-1080 with NaV1.7 VSDII peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.965, 78.965, 147.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.650 - 1.620 |
| R-factor | 0.1942 |
| Rwork | 0.194 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.670 | 1.678 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.107 | 0.107 |
| Number of reflections | 59782 | 59782 |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2M potassium thiocyanate, 25% W/V Polyethylene glycol 3,350 |
