8YHV
Structure of the VP40 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.031, 90.812, 49.220 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 45.447 - 2.350 |
| Rwork | 0.174 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.076 | 0.616 |
| Number of reflections | 11721 | 1136 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Magnesium chloride hexahydrate 0.1M Tris pH8.5, 25% PEG4000 |
