8YHN
Crystal structure of Cytochrome P450 107P2 from streptomyces avermitilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 288.15 |
| Detector technology | PIXEL |
| Collection date | 2023-07-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.128, 66.440, 111.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.209 - 1.986 |
| Rwork | 0.223 |
| R-free | 0.27210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.463 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.209 | 5.179 |
| High resolution limit [Å] | 1.986 | 1.986 |
| Rmeas | 1.880 | |
| Number of reflections | 31112 | 1390 |
| <I/σ(I)> | 1.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 17.5% PEG 3350, 0.2M Magnesium acetate tetrahydrate, pH 7.5 |
