8YHI

The Crystal Structure of SHP1 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95373
Spacegroup name	P 21 2 21
Unit cell lengths	45.302, 56.498, 110.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.488 - 1.750
Rwork	0.193
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.087
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.088	0.975
Number of reflections	29341	1593
<I/σ(I)>	16.4
Completeness [%]	99.9
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Hepes pH7.5, 2% PEG 400, 2.0M (NH4)2SO4