8YHH

The Crystal Structure of Mitotic Kinesin Eg5 from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	51.654, 78.254, 93.467
Unit cell angles	90.00, 94.51, 90.00

Refinement procedure

Resolution	46.590 - 1.950
Rwork	0.214
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.590	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	51970	3726
<I/σ(I)>	13.7
Completeness [%]	96.1
Redundancy	5.4
CC(1/2)	0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	19% PEG3350, 0.1M MES (pH 5.2), and 200 mM NaNO3