8YH1
Crystal structure of Thermus thermophilus UMP kinase complexed with a phosphoryl group acceptor and donor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-19 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 150.217, 232.197, 284.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.320 - 2.600 |
| R-factor | 0.1808 |
| Rwork | 0.180 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.320 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.047 | 0.226 |
| Number of reflections | 152039 | 15086 |
| <I/σ(I)> | 39.9 | 2.66 |
| Completeness [%] | 100.0 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1 M MES, 2.0 M ammonium sulfate, 10 mM cobalt chloride |
