8YGZ

The Crystal Structure of TGF beta R2 kinase domain from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.18064
Spacegroup name	P 21 21 21
Unit cell lengths	61.090, 75.020, 77.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 2.100
Rwork	0.195
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	21483	1719
<I/σ(I)>	19.4
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Li2SO4, 0.1M Tris-HCl pH8.5, 30% PEG4,000