8YGX
Structure of the PYK2 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.253, 94.514, 42.668 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 42.686 - 2.000 |
| Rwork | 0.207 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.686 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.181 | 0.479 |
| Number of reflections | 18990 | 1444 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 95.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M MgCl2, 0.1M Bis-Tris pH5.5-5.9, 19-29% PEG3,350,1mM TCEP |
