8YG2
Crystal structure of amyloidogenic peptide Piv-NFGAIL-NH2 from Islet Amyloid Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-21 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.040 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 4.864, 20.466, 20.855 |
| Unit cell angles | 90.00, 88.60, 90.00 |
Refinement procedure
| Resolution | 20.470 - 1.250 |
| Rwork | 0.174 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.733 |
| Data reduction software | DIALS (2.2.10) |
| Data scaling software | DIALS (2.2.10) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.470 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.084 | 0.155 |
| Number of reflections | 1063 | 55 |
| <I/σ(I)> | 59.11 | |
| Completeness [%] | 91.9 | 94.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | Formic acid |
