8YFN

Crystal structure of FIP200 claw in complex with TNIP1_FIR_pS123 peptide with an elongated C terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 43
Unit cell lengths	51.369, 51.369, 85.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.684 - 2.300
R-factor	0.1907
Rwork	0.188
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.871
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.320	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.122	0.858
Number of reflections	9872	482
<I/σ(I)>	11.6	2.6
Completeness [%]	99.9	99.8
Redundancy	10.4
CC(1/2)	0.998	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M imidazole malate, pH 5.5, 42% PEG 600