8YFM

Crystal structure of FIP200 claw/TNIP1_FIR_pS122

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	61.072, 61.072, 65.302
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.536 - 1.500
R-factor	0.1932
Rwork	0.193
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.540	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.041	0.548
Number of reflections	43527	2108
<I/σ(I)>	24.8	2.3
Completeness [%]	99.7
Redundancy	9.6
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	25% v/v PEG smear medium, 0.1M sodium cacodylate, 0.2M ammonium sulfate, pH 5.5