8YEW

TRIP4 ASCH domain in unliganded form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	29.387, 67.487, 73.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.630 - 1.900
R-factor	0.2091
Rwork	0.207
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.901
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.123	0.546
Rmeas	0.147	0.649
Rpim	0.079	0.344
Total number of observations	33045	4942
Number of reflections	11259	1656
<I/σ(I)>	5.7	2.6
Completeness [%]	94.9
Redundancy	2.9	3
CC(1/2)	0.992	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M Li2SO4, 0.1 M Tris HCl (pH 8.5), and 30% (w/v) polyethylene glycol (PEG) 4,000.