8YEQ

Crystal structure of L7/L12 Ribosomal Protein from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 160 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-08
Detector	DECTRIS PILATUS3 R 1M
Wavelength(s)	1.36
Spacegroup name	P 2 21 21
Unit cell lengths	25.931, 47.282, 61.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.900 - 1.500
R-factor	0.1674
Rwork	0.166
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.900	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.024	0.093
Rmeas	0.026	0.103
Rpim	0.008	0.042
Number of reflections	12351	546
<I/σ(I)>	55.1	13.1
Completeness [%]	97.5	85.4
Redundancy	9.9
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Ammonium Acetate pH 7.0, 25% PEG 8000 25% Ethylene Glycol