8YEB
Crystal structure of L-ribulose 3-epimerase from Arthrobacter globiformis M30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.095, 148.264, 136.707 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.200 |
| R-factor | 0.20266 |
| Rwork | 0.201 |
| R-free | 0.23972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.120 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 | 0.518 |
| Rmeas | 0.094 | 0.622 |
| Rpim | 0.050 | 0.339 |
| Number of reflections | 258486 | 25340 |
| <I/σ(I)> | 10.09 | 1.93 |
| Completeness [%] | 99.1 | 97.5 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.995 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 200mM Ammonium acetate, 100mM BIS-TRIS pH 5.5 |
