8YE0

Crystal structure of KgpF prenyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.08
Spacegroup name	P 1
Unit cell lengths	45.681, 69.344, 86.426
Unit cell angles	109.11, 93.10, 104.89

Refinement procedure

Resolution	43.640 - 1.940
R-factor	0.1922
Rwork	0.191
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.722
Data reduction software	XDS
Phasing software	MrBUMP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.640	1.980
High resolution limit [Å]	1.940	1.940
Number of reflections	69214	4404
<I/σ(I)>	7.7
Completeness [%]	97.6
Redundancy	3.7
CC(1/2)	0.967	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	PEG2000MME, Bis-Tris, Magnesium chloride