8YDN
Crystal structure of a STING from C. gigas in complex with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-01-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 104.400, 104.400, 96.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.990 - 2.040 |
| R-factor | 0.2341 |
| Rwork | 0.233 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.544 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.990 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.187 | |
| Rmeas | 0.191 | |
| Rpim | 0.038 | |
| Number of reflections | 17362 | 1278 |
| <I/σ(I)> | 21.38 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 25 | |
| CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 5% PEG 400, 0.1 M MES pH 6.5, 2.0 M ammonium sulfate |
