8YDK

Crystal structure of a novel design for RSV F protein in pre-fusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	149.237, 64.912, 109.935
Unit cell angles	90.00, 130.28, 90.00

Refinement procedure

Resolution	32.760 - 2.100
R-factor	0.2216
Rwork	0.220
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	46587	4613
<I/σ(I)>	16.5	1.7
Completeness [%]	100.0
Redundancy	3.8
CC(1/2)	0.997	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	291	0.1 M phosphate/citrate (pH 4.2), 40 % v/v PEG 300