8YD8

Structure of FADD/Caspase-8/cFLIP death effector domain assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-26
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 21 21 21
Unit cell lengths	113.170, 149.680, 175.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.090 - 3.110
R-factor	0.2015
Rwork	0.200
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.435
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.090	30.090	3.210
High resolution limit [Å]	3.100	6.660	3.100
Rmerge	0.078	0.042	0.917
Rmeas		0.046	1.012
Rpim		0.019	0.423
Number of reflections	53985	5681	5185
<I/σ(I)>	9
Completeness [%]	99.7	99.6	97.5
Redundancy	6.1	5.8	5.6
CC(1/2)		0.998	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	HEPES, PEG 8000, TBG, TCEP, sodium chloride