8YBG

Crystal structure of lysozyme by serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	299
Detector technology	PIXEL
Collection date	2022-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	78.500, 78.500, 38.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.510 - 1.550
R-factor	0.186
Rwork	0.182
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.885
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.360	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	17968	1755
<I/σ(I)>	8.99
Completeness [%]	100.0
Redundancy	956.5
CC(1/2)	0.996	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	sodium acetate, sodium chloride, PEG 8000