8YBF

Crystal structure of canine distemper virus hemagglutinin

Replaces:  8IAY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	86.156, 86.156, 302.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.670 - 3.100
R-factor	0.2664
Rwork	0.264
R-free	0.30750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.160
High resolution limit [Å]	3.000	3.000
Rmerge		1.248
Rmeas	0.173
Rpim	0.069	0.492
Number of reflections	23165	2276
<I/σ(I)>	13.4	2.5
Completeness [%]	99.6
Redundancy	6.1
CC(1/2)		0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	20mM Tris-HCl pH 8.0, 100mM NaCl