8YAV

Crystal structure of glucose 1-dehydrogenase from Limosilactobacillus fermentum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	57.851, 58.079, 73.072
Unit cell angles	70.58, 83.46, 78.36

Refinement procedure

Resolution	42.970 - 1.750
R-factor	0.1898
Rwork	0.189
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7p7y
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.970	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.066	0.491
Rmeas	0.083
Number of reflections	85496	4143
<I/σ(I)>	14	3
Completeness [%]	96.7	89.2
Redundancy	5.8	5.3
CC(1/2)	0.998	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1M MES pH 6.0, 0.05M CaCl2, 43% PEG 200