8Y9W
CRYSTAL STRUCTURE OF A LYSOZYME FROM HEN EGG WHITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17UM |
| Synchrotron site | SSRF |
| Beamline | BL17UM |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.161, 77.161, 36.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.580 - 1.900 |
| R-factor | 0.2052 |
| Rwork | 0.200 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 0.820 |
| Rmeas | 0.104 | 0.866 |
| Rpim | 0.025 | 0.271 |
| Total number of observations | 156226 | 5781 |
| Number of reflections | 9250 | 587 |
| <I/σ(I)> | 16.6 | 1.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 16.9 | 9.8 |
| CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 300 | 0.1 M sodium acetate, 6% sodium chloride |
