8Y9T

Crystal structure of nanobody MY6322 bound to human serum albumin (HSA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	C 1 2 1
Unit cell lengths	167.951, 73.699, 86.008
Unit cell angles	90.00, 118.74, 90.00

Refinement procedure

Resolution	75.410 - 2.400
R-factor	0.26628
Rwork	0.263
R-free	0.32447
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS (0.7.7)
Data scaling software	DIALS (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.410	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.508
Rpim	0.052	0.434
Number of reflections	35902	2440
<I/σ(I)>	9.4	1.5
Completeness [%]	99.2	91.6
Redundancy	5.4	3.1
CC(1/2)	0.991	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M Sodium acetate trihydrate pH 4.6, 1.8 M Ammonium sulfate