8Y98

Crystal structure of a heterooligomeric aminotransferase from Serratia sp. ATCC 39006, PPE-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-02-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.978, 181.170, 182.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.860 - 2.310
R-factor	0.19486
Rwork	0.193
R-free	0.22318
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	2.360
High resolution limit [Å]	2.310	2.310
Rmerge	0.114	0.922
Rmeas	0.124	1.008
Rpim	0.047	0.399
Total number of observations	520607	27335
Number of reflections	77803	4444
<I/σ(I)>	14	1.9
Completeness [%]	99.5
Redundancy	6.7	6.2
CC(1/2)	0.998	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	293	dipotassium hydrogenphosphate, sodium dihydrogenphosphate, lithium sulfate, CAPS