8Y8Y

Crystal structure of the PTPN21 FERM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	60.789, 113.843, 47.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.550 - 2.099
R-factor	0.2049
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.837
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.099	2.099
Rmerge	0.066	0.353
Number of reflections	20123	959
<I/σ(I)>	22
Completeness [%]	99.1
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.04 M citric acid, 0.04 M Bis-tris-propane pH 5.0, 13.8% polyethylene glycol 3350