8Y7V

Crystal structure of CP2c/LSF DNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	57.093, 57.093, 130.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.610 - 1.600
R-factor	0.1941
Rwork	0.192
R-free	0.22940
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.918
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	28993	2775
<I/σ(I)>	31.69	8.31
Completeness [%]	98.7
Redundancy	15.6
CC(1/2)	0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.005 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0, 25% v/v Polyethylene glycol monomethyl ether 550