8Y7P

Crystal structure of human GFOD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	H 3
Unit cell lengths	184.940, 184.940, 58.073
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.910 - 2.600
R-factor	0.1991
Rwork	0.197
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q9NXC2
RMSD bond length	0.003
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.010	2.650
High resolution limit [Å]	2.600	2.600
Rmerge	0.076	0.875
Number of reflections	22586	1127
<I/σ(I)>	11.6	1.7
Completeness [%]	99.1
Redundancy	4.3	4.3
CC(1/2)	0.998	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Hepes, pH 7.5, 2% (v/v) Tacsimate, and 20% (w/v) PEG 3,350