8Y7G

Crystal structure of the Marinitoga sp. Csx1-Crn2 H495A mutant in complex with cyclic-tetraadenylate (cA4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	73.496, 119.564, 196.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.790 - 3.150
R-factor	0.2168
Rwork	0.212
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.377
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.790	3.270
High resolution limit [Å]	3.150	3.150
Number of reflections	30656	30656
<I/σ(I)>	13.88
Completeness [%]	99.7
Redundancy	13.2
CC(1/2)	0.997	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol 4000