8Y76

Crystal structure of the SAM domain of L3MBTL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 65
Unit cell lengths	51.820, 51.820, 49.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.910 - 1.990
R-factor	0.1719
Rwork	0.169
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.780
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.990	1.990
Rmerge	0.137	0.719
Number of reflections	5315	252
<I/σ(I)>	30.6
Completeness [%]	100.0
Redundancy	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4 M Sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0 and 0.5%(w/v) Polyethylene glycol 10000