8Y6Z

Crystal structure of the Marinitoga sp. Csx1-Crn2 fusion ribonuclease of type III CRISPR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 62 2 2
Unit cell lengths	230.330, 230.330, 59.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.390 - 3.650
R-factor	0.2125
Rwork	0.211
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.674
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.390	3.780
High resolution limit [Å]	3.650	3.650
Rmerge	0.153
Number of reflections	10761	1046
<I/σ(I)>	22.3
Completeness [%]	99.8
Redundancy	34.1
CC(1/2)		0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium acetate trihydrate pH 4.6, 8% w/v Polyethylene glycol 4000