8Y54

Three-dimensional structure of homo-dimer of cystathione beta lyase from lactobacillus delbrueckii(LdPatB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-12-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978610
Spacegroup name	C 1 2 1
Unit cell lengths	112.470, 91.060, 80.870
Unit cell angles	90.00, 125.27, 90.00

Refinement procedure

Resolution	38.950 - 1.780
Rwork	0.207
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.950	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.079	1.170
Number of reflections	63450	4699
<I/σ(I)>	13.2
Completeness [%]	99.6
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Magnesium formate dihydrate, Polyethylene glycol 3350