8Y3Z

VcFadRqm, Genetically engineered mutants of Vibrio cholerae fadR, in Complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 43 21 2
Unit cell lengths	99.303, 99.303, 225.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.500
R-factor	0.2349
Rwork	0.234
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.196
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.500	2.600
Rmerge	0.012
Number of reflections	39938	35669
<I/σ(I)>	21.3
Completeness [%]	99.9	99.9
Redundancy	2.52
CC(1/2)		0.010

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL	7.5	289.15	16%(w/v)PEG8000,40mM KH2PO4,20%(v/v) Glycerol