8Y30

Crystal structure of Staphylococcus aureus RecJ protein in complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 32
Unit cell lengths	199.313, 199.313, 62.764
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 - 2.800
R-factor	0.2473
Rwork	0.246
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.513
Data reduction software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.660	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.068	0.576
Number of reflections	68089	9925
<I/σ(I)>	10.6	2.8
Completeness [%]	98.9
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	20% peg 3350, 0.1 M (NH4)2SO4, 0.05 M MgSO4, pH 6.5