8Y14
Beta-catenin Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-06-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.850, 103.150, 185.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.198 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | HKL-2000 (4.5) |
| Data scaling software | HKL-2000 (4.5) |
| Phasing software | PHASES (1.2) |
| Refinement software | PHENIX ((1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 13374 | 13378 |
| <I/σ(I)> | 26.5 | |
| Completeness [%] | 92.5 | 86 |
| Redundancy | 7.3 | |
| CC(1/2) | 0.980 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 14% PEG 3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol |
