8Y0P

Beta-Catenin in complex with peptide 301

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.921, 100.758, 187.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.620
R-factor	0.24061
Rwork	0.239
R-free	0.27905
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.941
Data reduction software	HKL-2000 (4.5)
Data scaling software	HKL-2000 (4.2)
Phasing software	PHASER (2.0)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.620	2.620
Rpim	0.044
Number of reflections	37171	50
<I/σ(I)>	26.5
Completeness [%]	99.3
Redundancy	7.3
CC(1/2)	0.994	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	14% PEG 3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol