8Y0G

Human beta-catenin crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-05-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	63.842, 103.434, 186.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.238
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000 (4.2)
Data scaling software	HKL-2000 (4.2)
Phasing software	PHASER (2.0)
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rpim	0.030	0.030
Number of reflections	21671	1867
<I/σ(I)>	12.5
Completeness [%]	99.3
Redundancy	3.6
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Apo crystals of beta-Catenin were grown from 14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline Crystal were transferred into soaking solution and incubated at RT for 1 days. Crystals were transferred into cryoprotectant (14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol), and then flash frozen in liquid nitrogen.