8XZX
Structure of an engineered xylanase Xyl-1 M4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-01 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.633, 52.037, 52.232 |
| Unit cell angles | 96.91, 102.49, 107.51 |
Refinement procedure
| Resolution | 37.290 - 2.300 |
| R-factor | 0.16493 |
| Rwork | 0.162 |
| R-free | 0.23142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.503 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.290 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.229 |
| Number of reflections | 18177 | 897 |
| <I/σ(I)> | 15.59 | 4.15 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 6.52 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 295 | 0.2 M sodium formate, 0.1 M Bis-Tris propane 6.5, 20% PEG 3350 |
