8XZ5

SARS-CoV-2 S and moesin complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 43
Unit cell lengths	76.204, 76.204, 55.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.102 - 1.800
R-factor	0.2056
Rwork	0.204
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.725
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.800	1.800
Number of reflections	25653	3994
<I/σ(I)>	21.6
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.850	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Tris, pH 7.4, 0.2 M NH4Ac, and 21% PEG 3350