8XZ4

SARS-CoV-2 S and radixin complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 43
Unit cell lengths	77.375, 77.375, 58.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.688 - 2.129
R-factor	0.221
Rwork	0.216
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.647
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.100	2.100
Number of reflections	19283	1389
<I/σ(I)>	21.1
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.800	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	75 mM Sodium acetate, 0.1 M Tris-HCl, pH 7.4, 5.5% PEG 3350